新品速遞丨2022年8 月上市的經嚴格驗證的全新抗體、試劑及試劑盒
Osteopontin/SPP1 (E9Z1D) Rabbit mAb #88742
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使用 Osteopontin/SPP1 (E9Z1D) Rabbit mAb（上圖）或 β-Actin (D6A8) Rabbit mAb #8457（下圖）對未經處理 (-) 或經 λ 磷酸酶處理 (+) 的 RAW 264.7 和 Neuro-2a 細胞的提取物進行蛋白質印跡分析。
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對 RAW 264.7 細胞提取物中的骨橋蛋白/SPP1 蛋白進行免疫沉澱分析。泳道 1 為 10% 輸入，泳道 2 為 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900，泳道 3 為 Osteopontin/SPP1 (E9Z1D) Rabbit mAb。使用 Osteopontin/SPP1 (E4O2F) Rabbit mAb #27927 進行蛋白質印跡分析。Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) #93702 用作二抗。
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使用 Osteopontin/SPP1 (E9Z1D) Rabbit mAb 對石蠟包埋的小鼠卵巢組織進行免疫組織化學分析。
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使用 Osteopontin/SPP1 (E9Z1D) Rabbit mAb 對石蠟包埋的小鼠胰腺組織進行免疫組織化學分析。
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使用 Osteopontin/SPP1 (E9Z1D) Rabbit mAb 對石蠟包埋的小鼠腦的腦橋區組織進行免疫組織化學分析。
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使用 Osteopontin/SPP1 (E9Z1D) Rabbit mAb（頂部圖）或 Osteopontin/SPP1 Rabbit mAb（底部圖）對石蠟包埋的 renca 同基因腫瘤組織（左圖）、4T1 同基因腫瘤組織（中圖）或小鼠肝臟組織（右圖）進行免疫組織化學分析。這兩種抗體均可檢測小鼠骨橋蛋白/SPP1 上的獨特、非重疊表位。使用兩種抗體獲得的相似染色模式有助於確認染色特異性。
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與濃度匹配的 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（右圖）比較，使用 Osteopontin/SPP1 (E9Z1D) Rabbit mAb（左圖）對石蠟包埋的小鼠腎臟組織進行免疫組織化學分析。
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使用 Osteopontin/SPP1 (E9Z1D) Rabbit mAb 對石蠟包埋的 RAW 264.7 細胞沉澱物（左圖，陽性）或 Neuro-2a 細胞沉澱物（右圖，陰性）進行免疫組織化學分析。
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對用 Osteopontin/SPP1 (E9Z1D) Rabbit mAb（左圖，綠色）標記的固定冷凍小鼠腎臟組織進行共聚焦免疫螢光分析。隨後將游離的二抗結合位點用 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 封閉，之後用 Ras (E4K9L) Rabbit mAb (Alexa Fluor® 488 Conjugate) #51813（右圖，紅色）和 DAPI #4083（右圖，藍色）進行共標記。
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對用 Osteopontin/SPP1 (E9Z1D) Rabbit mAb（左圖，綠色）標記的固定冷凍小鼠丘腦組織進行共聚焦免疫螢光分析。然後將游離二抗結合位點用 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 封閉，之後用 Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #78060（右圖，紅色）和 DAPI #4083（右圖，藍色）共標記。
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使用 Osteopontin/SPP1 (E9Z1D) Rabbit mAb（綠色）和 DAPI #4083（藍色）對 RAW 264.7 細胞（左圖，陽性）和 Neuro-2a 細胞（右圖，陰性）進行共聚焦免疫螢光分析。用 Brefeldin A #9972（100 ng/mL，90 分鐘）處理細胞以抑制蛋白質分泌。
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Osteopontin/SPP1兔單克隆抗體用於識別內源水平的骨橋蛋白/SPP1 總蛋白。骨橋蛋白是一種在多種細胞類型中表達的高度磷酸化分泌型糖蛋白。分泌型磷蛋白 1 (SPP1) 基因編碼的骨橋蛋白最初從大鼠骨肉瘤細胞系中分離並克隆 【1】。骨橋蛋白是一種高度帶負電荷的蛋白質，該蛋白質可能調節骨骼和其他組織中的胞外基質周轉和重塑 【2】。骨橋蛋白還具有三個高度保守的受體結合結構域，也稱為精氨酸-甘氨酸-天冬氨酸 (RGD) 結構域。
關鍵詞：纖維化，硬化，阿爾茨海默病，炎症
查看明星產品詳情：
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貨號 | 產品名稱 | 應用 | 反應性 |
Adhesion / ECM | |||
CD54/ICAM-1 (E3Q9N) XP® Rabbit mAb (BSA and Azide Free) | WB, IHC-Bond, IHC-P | H, Mk | |
Decorin (E2N2C) XP® Rabbit mAb | WB, IHC-P | H | |
ECM1 (E3B9A) Rabbit mAb | WB, IF-IC | H | |
Osteopontin/SPP1 (E9Z1D) Rabbit mAb | WB, IP, IHC-P, IF-F, IF-IC | M | |
ZO-1 (6B6E4) Rat mAb | WB, IF-IC | H, Dg | |
Apoptosis | |||
Bak (D4E4) Rabbit mAb (Alexa Fluor® 488 Conjugate) | FC-FP | H, M, R, Mk | |
Cleaved Gasdermin D (Gly277) Antibody | WB | M | |
Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Pacific Blue™ Conjugate) | FC-FP | H, Mk | |
Takinib | - | - | |
Autophagy | |||
CDDO-Me | - | - | |
Phospho-SQSTM1/p62 (Ser366) Antibody | WB | H, M, R | |
TAK-242 | - | - | |
Cell Cycle / Checkpoint Control | |||
p63 (D9L7L) XP® Rabbit mAb (BSA and Azide Free) | WB, IHC-P | H | |
PathScan® Phospho-PLK1 (Thr210) Sandwich ELISA Kit | ELISA | H | |
Chromatin Regulation / Nuclear Function | |||
Symmetric Di-Methyl Histone H3 (Arg2) (E8V5Q) Rabbit mAb | WB, IP | H, M, R, Mk | |
FastScan™ Tri-Methyl-Histone H3 (Lys27) ELISA Kit | ELISA | H, M, R, Mk | |
LYAR (E6H1A) Rabbit mAb | WB | H, M, R | |
MYST Antibody Sampler Kit | - | - | |
TASOR (E6M2Y) Rabbit mAb | WB | H, Mk | |
TET1 (E2J2D) Rabbit mAb | WB, C&R | M | |
UTY (E4X6V) Rabbit mAb | WB, IP | M | |
ZBTB24 (E5D6G) Rabbit mAb | WB, IP, ChIP | H, M, R, Mk | |
ZNF217 (E6X4R) Rabbit mAb | WB, IP, IF-IC, ChIP | H | |
Cytoskeletal Signaling | |||
NCKIPSD (E6Z3N) Rabbit mAb | WB, IP | H, M | |
Developmental Biology | |||
β-Catenin (D10A8) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) | IF-F, IF-IC, FC-FP | H, M, R, Mk, (Z, B, Pg, Hr, GP) | |
β-Catenin (D10A8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) | IF-F, IF-IC, FC-FP | H, M, R, Mk, (Z, B, Pg, Hr, GP) | |
β-Catenin (D10A8) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) | IF-F, IF-IC, FC-FP | H, M, R, Mk, (Z, B, Pg, Hr, GP) | |
β-Catenin (D10A8) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) | IF-F, IF-IC, FC-FP | H, M, R, Mk, (Z, B, Pg, Hr, GP) | |
Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (Pacific Blue™ Conjugate) | FC-FP | H, M, R, Mk, Z, Dg | |
Hedgehog Signaling Antibody Sampler Kit | - | - | |
Small Cell Lung Cancer Biomarker Antibody Sampler Kit | - | - | |
Immunology and Inflammation | |||
Aiolos (D1C1E) Rabbit mAb (Pacific Blue™ Conjugate) | FC-FP | H, M | |
C9orf7/CACFD1 (E6E3J) Rabbit mAb | WB, IP | H | |
CD5 (E8X3S) XP® Rabbit mAb (BSA and Azide Free) | WB, IHC-Bond, IHC-P | H | |
CXCL9/MIG (E6Z5W) Rabbit mAb (BSA and Azide Free) | WB, IHC-P | H | |
GITR (D9I9D) Rabbit mAb (IHC Preferred) (BSA and Azide Free) | WB, IHC-Bond, IHC-P | H, (Mk) | |
Goat Anti-Mouse IgM, F(ab')2 Antibody (Low Endotoxin, Azide-free) | FUNC | M | |
Helios (D8W4X) XP® Rabbit mAb (Pacific Blue™ Conjugate) | FC-FP | H, M | |
Helios (E4L5U) Rabbit mAb (BSA and Azide Free) | WB, IHC-Bond, IHC-P | H, M, (B) | |
Human Reactive PANoptosis Antibody Sampler Kit | - | - | |
IFITM2/IFITM3 (E5F8C) Rabbit mAb | WB, IP | H | |
IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) | FC-L | M | |
IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) | IF-F | M | |
IL-8 (E5F5Q) XP® Rabbit mAb (BSA and Azide Free) | WB, IHC-P, FC-FP | H | |
Phospho-Jak Family (Tyr1007/1008) (E9E4O) Rabbit mAb | WB | H, M | |
α-2-Macroglobulin (E4H4U) Rabbit mAb | WB, IP, IF-IC | H | |
α-2-Macroglobulin (E6N7X) Rabbit mAb | WB, IP, IHC-P | H | |
MAGE-A4 (E7O1U) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) | FC-FP | H | |
MHC Class II (LGII-612.14) Mouse mAb (PE Conjugate) | FC-L | H | |
NF-κB p65 (D14E12) XP® Rabbit mAb (Pacific Blue™ Conjugate) | FC-FP | H, M, R, Hm, Mk, Dg | |
TNFRSF8/CD30 (E4L4I) XP® Rabbit mAb (BSA and Azide Free) | WB, IHC-P | H, M | |
TNFRSF13C/BAFF-R (E3C1Q) XP® Rabbit mAb (BSA and Azide Free) | WB, IHC-P | H, (Pg) | |
Metabolism | |||
FIS1 (E3K9O) Rabbit mAb | WB, IP, IF-IC | H, M, R | |
Perilipin-2 (E6G6M) Rabbit mAb | WB, IP, IF-IC | H | |
TIMM17A (E6F3I) Rabbit mAb | WB, IP | H, M, R | |
Neuroscience | |||
ß-Amyloid (D54D2) XP® Rabbit mAb (BSA and Azide Free) | WB, IF-F | H | |
ApoE (pan) (D7I9N) Rabbit mAb (BSA and Azide Free) | WB, IHC-P, IF-IC | H | |
CD39/NTPDase 1 (E2X6B) XP® Rabbit mAb (Mouse Specific) (BSA and Azide Free) | WB, IHC-P | M | |
GFAP (GA5) Mouse mAb (BSA and Azide Free) | WB, IHC-P, IF-F, IF-IC, FC-FP | H, M, R | |
NCAM-L1 (D5D3K) Rabbit mAb (BSA and Azide Free) | WB, IHC-P | H, Mk | |
Neurofilament-L (C28E10) Rabbit mAb (BSA and Azide Free) | WB, IHC-P, IF-F | H, M, R | |
PSD95 (7E3) Mouse mAb (BSA and Azide Free) | WB, IF-F, IF-IC | H, M, R | |
S100B (E7C3A) Rabbit mAb (BSA and Azide Free) | WB, IHC-P, IF-F, IF-IC | H, M, R | |
Tau (Tau46) Mouse mAb (BSA and Azide Free) | WB, IHC-P | H, M, R, (B) | |
Phospho-Tau (Thr181) (D9F4G) Rabbit mAb (BSA and Azide Free) | WB, IHC-P, IF-F | H, M, R | |
Phospho-Tau (Ser416) (D7U2P) Rabbit mAb (BSA and Azide Free) | WB, IHC-P | H, M, R | |
TREM2 (D8I4C) Rabbit mAb (BSA and Azide Free) | WB, IF-IC | H | |
Nuclear Receptor Signaling | |||
PhosphoPlus® Androgen Receptor (Ser258) Antibody Duet | - | - | |
Steroid Hormone Receptor Antibody Sampler Kit | - | - | |
PI3K / Akt Signaling | |||
RapaLink-1 | - | - | |
Protein Folding and Trafficking | |||
GCN2 (E9A9R) Rabbit mAb | WB, IP | H, M, R | |
Translational Control | |||
N1-Methyladenosine (m1A) (E8S7H) Rabbit mAb | R Dot Blot | All | |
S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 700 Conjugate) | IF-F, IF-IC, FC-FP | H, M, R, Mk, Dm | |
Ubiquitin and Ubiquitin-like proteins | |||
OTULIN (E3B8L) Rabbit mAb | WB, IP | H, M, R, (Pg) | |
Related Products | |||
JC-1 | - | - | |
Mithramycin A | - | - | |
Puromycin Dihydrochloride | - | - | |
在此
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【參考文獻】
1. Oldberg, A. et al. (1986) Proc Natl Acad Sci U S A 83, 8819-23.
2. Kazanecki, C.C. et al. (2007) J Cell Biochem 102, 912-24.
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